Through the proposed method GC content of human DNA sequence have been calculated. The GC content plays a major role in disease prediction. Normally in a human genome the GC content is 35% to 60% , if found less than 35% then it indicates about some deficiency diseases like essential amino acid deficiency disease ( mainly Alanine, proline, glycine); and if this content is found more than 60%, then it can be indication of some chromosomal or genetic diseases. So, based on the report of GC content a human can take some precautions to eradicate the probability of happening these kind of diseases.

Alanine, Cytosine, DNA, Guinine, Glycine, Proline

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